Guide Molecular Methods in Developmental Biology. Xenopus and Zebrafish

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Contents:
  1. Wholemount In Situ Hybridization of Xenopus and Zebrafish Embryos | Springer Nature Experiments
  2. Molecular & Developmental Biology (BIOL3530)
  3. View Molecular Methods In Developmental Biology Xenopus And Zebrafish 1999

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Lecture 6 Organizer Axis Specification

Help a Privacy and be your bots with several ia. Motor entries -- Motors -- Exhaust registrar -- new Thanks. B , The transplanted GFP-labeled cells from the transgenic embryo carrying gfap:gfp construct are shown in the forebrain of the donor embryo. The red staining corresponds to alpha-tubulin. The image is reproduced from Figure 2 in [ 7 ]. Temporally-controlled site-specific recombination in zebrafish.

Wholemount In Situ Hybridization of Xenopus and Zebrafish Embryos | Springer Nature Experiments

Large-scale enhancer detection in the zebrafish genome. Multicolor fluorescent in situ hybridization to define abutting and overlapping gene expression in the embryonic zebrafish brain. Neural Dev. Inoue D, Wittbrodt J. One for all--a highly efficient and versatile method for fluorescent immunostaining in fish embryos. Reciprocal endoderm-mesoderm interactions mediated by fgf24 and fgf10 govern pancreas development.

Tissue targeted embryonic chimeras: zebrafish gastrula cell transplantation. J Vis Exp. Single-cell reconstruction of developmental trajectories during zebrafish embryogenesis. The dynamics of gene expression in vertebrate embryogenesis at single-cell resolution. Gerlai R. Reproducibility and replicability in zebrafish behavioral neuroscience research. Pharmacol Biochem Behav. The Zebrafish Book. Available from: zfin.

Molecular & Developmental Biology (BIOL3530)

Methods Mol Biol. Genome editing with RNA-guided Cas9 nuclease in zebrafish embryos. Cell Res. Nat Biotechnol. Do fish perceive anaesthetics as aversive?. Conditioned place avoidance of zebrafish Danio rerio to three chemicals used for euthanasia and anaesthesia. Microinjection of zebrafish embryos to analyze gene function. Available from: www. A zebrafish nanos-related gene is essential for the development of primordial germ cells.

Genes Dev. The zebrafish dorsal axis is apparent at the four-cell stage. A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo. Nat Commun.


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Jowett T, Lettice L. Whole-mount in situ hybridizations on zebrafish embryos using a mixture of digoxigenin- and fluorescein-labelled probes. Trends Genet. Programmable in situ amplification for multiplexed imaging of mRNA expression. Kawakami K.

View Molecular Methods In Developmental Biology Xenopus And Zebrafish 1999

Tol2: a versatile gene transfer vector in vertebrates. Genome Biol. Efficient transposition of the Tol2 transposable element from a single-copy donor in zebrafish. AIBP-mediated cholesterol efflux instructs hematopoietic stem and progenitor cell fate. MAZe: a tool for mosaic analysis of gene function in zebrafish.